These data together strongly suggested that rSV40 vectors integrate and that they do so in both cycling and non-cycling cell populations, both in vitro and in vivo. We then tested directly whether rSV40 DNAs were incorporated into cellular DNA. PCR analyses of genomic DNA in a transduced lymphocyte line yielded the predicted PCR product. That vector DNA inserted into the cell genome was demonstrated directly by two types of studies. Southern blot analysis of restricted and unrestricted high-molecular weight DNAs from cells transduced in vitro showed that almost all vector DNA was incorporated into chromosomal DNA within a few days of delivery. The detection of very small amounts of unintegrated vector DNA underscores the fact that practically all SV(BUGT) DNA integrated into the genome. Comparable Southern blot analyses performed on restricted DNA from livers transduced in vivo yielded a similar interpretation: rapid incorporation of rSV40 DNA into high-molecular weight cellular DNA (J.R.C. and D.S.S., unpublished data). A degree of incomplete digestion of the DNA notwithstanding, there is no other explanation for the observed release of SV(BUGT) restriction fragments from the genomic DNA, as seen in the smear obtained using NotI and in the patterns and sizes of bands obtained with NotI + Eco RI, other than that SV(BUGT) DNA integrated into the cellular genome.
Penetrating insight. Something we all require. Religion and real-world morality go hand-in-hand. I love it when prominent people reform. It’s terrible to see nastiness all the way to the top. Thune may set a good example which may shine for generations. ABN
Results: The analysis of claims data for 47,155 nine-year-old children revealed that: 1) vaccination was associated with significantly increased odds for all measured NDDs [neurodevelopmental disorders]; 2) among children born preterm and vaccinated, 39.9% were diagnosed with at least one NDD compared to 15.7% among those born preterm and unvaccinated (OR 3.58, 95% CI: 2.80, 4.57); and 3) the relative risk of ASD [autism spectrum disorder] increased according to the number of visits that included vaccinations. Children with just one vaccination visit were 1.7 times more likely to have been diagnosed with ASD than the unvaccinated (95% CI: 1.21, 2.35) whereas those with 11 or more visits were 4.4 times more likely to have been diagnosed with ASD than those with no visit for vaccination (95% CI: 2.85, 6.84).
Conclusions: These results suggest that the current vaccination schedule may be contributing to multiple forms of NDD; that vaccination coupled with preterm birth was strongly associated with increased odds of NDDs compared to preterm birth in the absence of vaccination; and increasing numbers of visits that included vaccinations were associated with increased risks of ASD.
A high-flying Silicon Valley couple has been arrested after allegedly being caught swindling over $60million in investors cash for their luxury lifestyle.
Alexander Beckman, 41, the founder and former CEO of AI firm GameOn, and his attorney wife Valerie Lau Beckman, 38, are accused of carrying out a ‘brazen and wide-ranging’ six-year fraud.
The couple face dozens of federal charges including securities fraud, conspiracy and identity theft, allegedly using investor cash to buy luxury homes, cars, and private schools for Alexander’s children.
Beckman made his name in Silicon Valley after founding GameOn in 2014, creating AI chatbot services for customers including professional sports teams and fashion retailers.
He was seen at galas rubbing shoulders with elites including Twitter founder Jack Dorsey, all the while allegedly stealing millions from investors and hiding it with fraudulent documents.
According to an indictment filed this week in San Francisco U.S. District Court, the scheme came tumbling down after GameOn’s board of directors found a company bank account meant to have over $11 million only contained 37 cents.
Miller is an example of how likeable Jews can be when they are on our side, or vociferously appear to be. I do not fully understand why Jews do not take a (real) Miller approach as displayed above and also do it across the board. Support the America you have tried to destroy rather than continue destroying it. I am serious, people would love you for it. ABN
1. Do you really want to see the JFK, RFK and MLK assassination files?
Here’s the direct way to get them using the plenary power of the Executive in combination with the Unitary application of govt recently affirmed by SCOTUS.
WARNING: This is extremely NON-Traditional.
2. Understanding the ordinary process of declassifying documents is a request and authorization to the executive officers and stakeholders of classified information; and understanding the current authorization is not ordinary because the intelligence community stakeholders are averse to the interests of the office of the president; here is a process to cut through the chaff and countermeasures.
The background here is that any unilateral declassification request, demand or authorization by President Trump puts him opposition to a variety of corrupt interests.
As a direct result the executive office of the president will be facing legal action, likely from unified democrats and republicans in the legislative branch. With that accepted, here is the most strategic approach.
3. President Trump informs the Office of the Director of National Intelligence, Tulsi Gabbard, that he wishes to have a full intelligence briefing on the following documents (more may be added), all documents are to be presented without a single redaction:
The full and complete records relating to the assassination of President John F. Kennedy.
The full and complete records relating to the assassination of Senator Robert F. Kennedy and the Reverend Dr. Martin Luther King, Jr.
The President selects a date for this briefing and through direct orders to his chief of staff, Susie Wiles and National Security Advisor, Mike Waltz, informs the Office of the Director of National Intelligence, Tulsi Gabbard, to advise and coordinate with all executive branch intelligence officials, who were/are stakeholders in the compartmented intelligence products as described above, of their request be present for the briefing.
The White House counsel’s office is not to be informed of the intent or purpose of the meeting; however, the Presidents’ White House counsel is requested to attend. Further, all of the compartmented intelligence is to be collectively assembled by the ODNI (Gabbard) into one volume of a singular Presidential Daily Briefing (PDB) although each set might be file boxes. There are to be eighteen printed copies of the PDB material assembled and secured for the briefing, post haste.
Additionally, the office of the president personally informs the ODNI (Gabbard) of the executives’ request to invite for the briefing each member of the legislative branch Intelligence Community oversight known as the Gang-of-Eight.
4. Immediately after the briefing by the executive level (cabinet) department officials, while remaining in a closed and classified session, the full and comprehensive content of this collective intelligence product will be discussed with the full assembly of the U.S. Legislative Branch Intelligence Oversight known as the Gang of Eight.
Therefore, National Security Advisor Mike Waltz is instructed to coordinate with the ODNI (Gabbard) for the attendance of the Gang of Eight: Speaker Mike Johnson, Minority leader Hakeem Jeffries, HPSCI Chairman Rick Crawford, HPSCI Ranking Member Jim Himes, Senate Majority Leader John Thune, Senate Minority Leader Chuck Schumer, SSCI Chairman Tom Cotton and SSCI Vice-Chair Mark Warner. [Topic “TBD”]
In order to facilitate the briefing. Each member of the participating group will be provided with one full printed copy of the material assembled by the ODNI during the briefing.
[Each of the participants carries the prerequisite clearances, legal and constitutional authority to engage with the classified documents according to their position and status. Only the executive can assemble the product for Go8 review and feedback]
At the conclusion of the briefing; and after hearing from, and engagement with, each of the participating members of the executive intelligence offices and duly authorized legislative oversight representatives; and after listening to their opinion as to the subject material discussed; the president announces to the fully assembled leadership of both the Executive branch (cabinet) and Legislative branch (Go8), it is his opinion the National Interests of the United States can best be served with the American people having a full, transparent and honest review of the material assembled and discussed.
American Buddhist Net 